INFLUENCE OF VITAMIN-A ON CORNEAL REGENERATION IN TADPOLES

Abstract

This study comprises of regeneration of cells of cornea. This study supports the possibility of their clinical use as a cell
source for reconstruction of the damaged corneal surface. For this experimental study, tadpoles were reared from eggs
collected from ponds in two developmental stages that are 3 toe stage and 5 toe stages. Tadpoles were anaesthetized. The
tadpoles were reared in conditioned tap water for the control group and exposed to 5 IU/ml vitamin A for experimental
groups tadpoles. Tadpoles were then subjected to operate their cornea and reared in vit-A solution. The amputated part or
tissue is then dehydrated, cleared and infiltrated with embedding material. The final embedding was done into wooden
block and then section was cut by microtomy. Slides were prepared and stained. Examinations of slides were done under
inverted microscope. Tadpoles treated with vit-A showed more corneal regeneration as compared to tadpoles reared in
water as control group. Therefore, vitamin –A has proved to be significant chemical model to induce and accelerate the
corneal regeneration.