ANALYTICAL FINGERPRINTING OF THE SIDDHA POLYHERBAL REMEDY LAVANGADHI VADAGAM (LV) USING FTIR, TLC/HPTLC AND ICP-OES –A QUALITY CONTROL APPROACH

Abstract

Siddha polyherbal formulations, rooted in traditional medicine, offer promising holistic management due to their synergistic bioactive constituents. However, standardization and quality control remain critical challenges for their wider acceptance. The present study focuses on the analytical fingerprinting of a Siddha polyherbal remedy Lavangadhi Vadagam (LV) using advanced instrumental techniques, namely Fourier Transform Infrared Spectroscopy (FTIR), High Performance Thin Layer Chromatography (HPTLC), and Inductively Coupled Plasma Optical Emission Spectrometry (ICP-OES). FTIR analysis was employed to identify functional groups and characterize major phytochemical classes present in the formulation. HPTLC profiling provided a comprehensive chromatographic fingerprint, enabling the detection and comparison of key bioactive compounds. ICP-OES analysis was conducted and to assess the presence of heavy metals, ensuring safety and compliance with pharmacopeial standards. The integration of these analytical techniques establishes a robust quality control framework for the Siddha formulation LV.