Standardization of Siddha Herbal Formulation “Kalladaippuku Kudineer Chooranam”

Abstract

Kalladaippuku Kudineer Chooranam is a Siddha herbal formulation traditionally used in the management of urolithiasis. The main aim of this study was to standardize the drug and to evaluate its microbial load and aflatoxin content in accordance with AYUSH protocols and WHO guidelines. Preliminary phytochemical screening revealed the presence of carbohydrates, flavonoids, quinones, and carboxylic acids. Physicochemical analysis indicated an acidic pH, with total ash value, acid-insoluble ash, and water-extractive values of 6.18%, 1.35%, and 11.59%, respectively. HPTLC fingerprinting showed seven peaks at 254 nm and eight peaks at 366 nm in the densitometric chromatogram. Aflatoxin levels were within the permissible limits, and no microbial contamination was detected. The findings of this study contribute to the quality assessment of the formulation and support its therapeutic potential in the management of urolithiasis.