Evaluation of Anti Diabetic Activity of Crateva Religiosa Bark Extract

Abstract

Diabetes mellitus remains a significant global health challenge, with increasing prevalence and complex treatment demands, particularly for Type 2 diabetes. This study investigates the in vitro anti-diabetic potential of Crateva religiosa bark extract, a medicinal plant traditionally used in Ayurveda and Unani systems. Methanolic extract of the bark was evaluated for its ?-amylase inhibitory activity and hydrogen peroxide (H?O?) scavenging capacity to elucidate mechanisms relevant to glycemic control and oxidative stress mitigation. Results demonstrated a dose-dependent inhibition of ?-amylase, indicating effective suppression of starch hydrolysis and potential attenuation of postprandial hyperglycemia. Remarkably, the extract exhibited superior antioxidant activity, with a 95.38% H?O? scavenging rate, outperforming standard antioxidants. These dual actions—enzyme inhibition and potent free radical neutralization-are likely attributable to the presence of bioactive phytochemicals such as flavonoids, tannins, alkaloids, and saponins. The findings underscore the therapeutic promise of C. religiosa as a multi-targeted natural agent for Type 2 diabetes management, addressing both metabolic dysregulation and oxidative stress, key contributors to disease progression. Future in vivo investigations and clinical evaluations are essential to validate efficacy, safety, and mechanistic pathways, paving the way for integration into complementary diabetes care paradigms.