Development And Validation Of HPLC Method For The Simultaneous Estimation Of Chlorthalidon And Metoprolol Succinate In Bulk And Dosage Form
Keywords:
Chlorthalidon, Metoprolol Succinate, Buffer pH 4.5, Methanol and AcetonitrileAbstract
A simple, accurate and precise High Performance Liquid Chromatographic (HPLC) method has been developed for simultaneous determination of Chlorthalidon and Metoprolol Succinate in bulk and dosage form. The method has been validated as per the guidelines of ICH. The separation is achieved on 250*4.6mm C18, 5micron (Hypersil BDS) column with flow rate 1.0 mL per minute in isocratic mode using Buffer pH 4.5: Methanol : acetonitrile 50:25:25) as mobile phase. Column oven temperature is maintained at 25°C and observations are recorded at 223 nm. The linearity range was found to be in the range of 5-15 ?g/ml for Chlorthalidon and 20-60 ?g/ml for Metoprolol Succinate. Correlation coefficient for calibration curve of Chlorthalidon and Metoprolol Succinate was found to be 0.998 and 0.999 espectively.
The method is simple, accurate, reproducible and short and can be used for the simultaneous analysis of Chlorthalidon and Metoprolol Succinate.
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