Analytical method development and validation of secnidazole in its pure and pharmaceutical dosage form by using uv-visible spectrophotometric method

Abstract

UV-spectrophotometry refers to absorption spectroscopy in the ultraviolet-visible spectral region. This method of analysis is gaining importance as it is rapid, simple, precise, less time consuming and accurate. The objective of present investigation was to develop an accurate, rapid and robust method for determination of  Secnidazole in API and in  pharmaceutical preparations by using UV spectrophotometric method. Secnidazole shows maximum absorbance at a wavelength of 305nm, which is used for this study. The method provides a linear response from a quantitation range of 06 µg/ml to 14 µg/ml in ethanol with regression equation y = 0.032x - 0.002 and r² 0.999 Interday precision and accuracy was found to be below 0.2 and above 99.00% respectively for the developed method. Thus the developed method suitably applied for regular quality control of Secnidazole in pure and pharmaceutical preparations.