DEVELOPMENT AND VALIDATION OF REVERSE PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHYMETHOD FOR SIMULTANEOUS ESTIMATION OF BETAHISTINE DIHYDROCHLORIDE AND PROCHLORPERAZINE MALEATE IN TABLET DOSAGE FORM

Abstract

This research manuscript describes simple, sensitive, precise, accurate, economic reverse phase high performanceliquid chromatography method for the simultaneous determination of Betahistine Dihydrochloride (BET) and Prochlorperazine Maleate (PRO) in Tablet dosage form. The sample was analyzed by reverse phase C18 column ( X terra C18, 150 mm × 4.6 mm, 5?m) asstationary phase; acetonitrile : methanol : phosphate buffer PH 4 (45 : 45 : 10 , v/v/v) as a mobile phase at a flow rate of 0.8 ml/min. Quantification was achieved with Photo Diode Array detector at 254 nm. The retention time for Betahistine Dihydrochloride was found to be 3.3 min and for Prochlorperazine Maleate was found to be 7.6 min. The linearity was obtained in theconcentration range of 2-30 ?g/ml and 2-30 ?g/ml for BET and PRO respectively. The method was successfully applied in analysis of tablet dosage form because no chromatographic interferences from formulation excipients were found. The method retained its accuracy and precision when the standard addition technique was applied.