Isolation of phytoconstituent from fruits of Gmelina arborea roxb

Abstract

The plant Gmelina arborea Roxb. has been traditionally used in India for several medicinal purposes like anthelmintic, diuretic, antibacterial, antipyretic, antioxidant and antidiabetic. The aim of the present study is to isolate the phytoconstituent from ethanolic fruits extracts of G. arborea. The G. arborea plant materials are rich source of flavonoid compounds and are responsible for various pharmacological activities.  The isolation of phytoconstituent was done from the ethanolic extract by the chromatographic method. The structure of the isolated compound was established on the basis of physical, chemical test and spectroscopic evidences such as IR, UV, H¹ NMR and C¹³ NMR and MS. A flavonoidal structure was isolated from the ethanolic extract of the plant in mobile phase of 5 % methanol in ethyl acetate (1:4). The yellow colored compound which solubilizes in methanol, chloroform and n-hexane and water having melting point was 177°C. It could be concluded that isolated compound may be quercetin (5,7-dihydroxy-4 methoxy flavone) which may be responsible for various pharmacological activities of the plants.