Effect of Macrotyloma uniflorum on free radicals and an-tioxidants in tissues of high fructose-fed rats
Keywords:
Antioxidants; fructose diet, insulin resistance, lipid peroxidation, liver, M. uniflorumAbstract
Feeding rats with high fructose, induces insulin resistance, hyperinsulinaemia, elevation of blood glucose level and impaired glucose tolerance. Oxidative stress plays a vital role in pathology associated with insulin resistance. The present study aimed to investigate the effects of Macrotyloma uniflorum (M. uniflorum) on the oxidant-antioxidant status in liver, kidney and heart of high fructose-fed diet (HFFD) rats. Male albino Wistar rats (160-180 g) were divided into six groups. Groups I and II received control diet (Group I served as normal control, group II received M.uniflorum (1000 mg/kg). Groups III-IV received HFFD (groups IV-VI received 250, 500 and 1000 mg/kg of M. uniflorum respectively). The HFFD fed rats showed increased levels of glucose, thiobarbituric acid reactive substances (TBARS), conjugated dienes (CD), lipid hydroperoxides (HP) and impaired antioxidant defense as evidenced by decreased in the activities of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx) glutathione reductase (GR) and the levels of reduced gluatathione (GSH). Treatment with M.uniflorum to the fructose-fed rats mitigated these alterations. M. uniflorum administration for 15 days decreased glucose levels; lipid peroxidation and restored the antioxidant potential. These findings support and strengthen the utility of M. uniflorum in the management of IR and associated pathology during diabetes.
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