Invitro Antiproliferative Effect Of Marine Macroalgae On Different Human Cancer Cell Lines
Keywords:
Macroalgae, Cancer, XTT assay, Seaweed. Turbinaria ornate, Cell linesAbstract
Natural compounds from marine ecosystem constitute the most promising source of novel bioactive compounds with potential function in human therapeutics and cancer treatment. Most importantly, marine algae were proved to be an important source of bioactive agents. The present study was aimed to evaluate the in vitro antiproliferative/anticancer properties of selected marine macroalgae extracts (n=34), derived from different species of brown and red marine macroalgae on lung cancer (NCI-H1975), prostate cancer (LnCaP), breast cancer (MCF7) and epidermoid cancer (A431) cell lines. The algae extracts were assayed for their anticancer properties on 4 human cancer cell lines using XTT cell proliferation assay. On screening of 34 algae extracts, the ethanol and water extract of brown algae Turbinaria ornata (S4 and S3) exhibited remarkably high anticancer activity on NCI-H1975 (lung) and LnCaP (prostate) cancer cells. Hence, the bioactive components from marine macroalgae might be used as potential therapeutic candidate against cancer.
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References
WHO, Cancer Fact sheet February 2017. http://www.who.int/mediacentre/factsheets/fs297/en/.
Torre, L.A., Bray, F., Siegel, R.L., Ferlay, J., Lortet-Tieulent, J., Jemal, A., 2015. Global cancer statistics 2012, CA. Cancer. J. Clin. 65, 87-108.
Kolanjinathan, K., Ganesh, P., Saranraj, P., 2014. Pharmacological Importance of Seaweeds. World. Journal. of Fish. and Marine. Sciences. 6 (1), 01-15.
Vasudevarao, B., Boominathan, M., 2016. Cytotoxic effect of methanol extracts of seaweeds. International. Journal. of Pharma. and Bio Sciences. 1, 98-105.
Blunden, G., 1993. Marine algae as sources of biologically active compounds. Inter. Discip. Sci. Rev. 18, 73-80.
Jin-Ching, L., Ming-Feng, H., Hurng-Wern, H., Fang-Rong, C., Chi-Chen, Y., Jen-Yang, T., Hsueh-Wei, C., 2013. Marine algal natural products with anti-oxidative, anti-inflammatory, and anti-cancer properties. Cancer. Cell. International. 13, 55.
Kokabi., Maryam., Yousefzadi., Morteza., Ali, A., 2013. Antioxidant Activity of Extracts of Selected Algae from the Persian Gulf, Iran. Journal. of the Persian. Gulf. 6, 45-50.
Suja, G., Vinoth K.T., Lakshmana, S.S., 2017. Screening of in vitro cytotoxic activity of brown seaweeds against hepato cellular carcinoma. Journal. of Applied. Pharmaceutical. Science. 7, 051-055.
Manilal, A., Sujith, S., Kiran, G.S., Selvin, J., Shikar, C., 2009. Cytotoxic potentials of red alga, laurencia brandenii collected from the Indian Coast. Global. J. Pharmacol. 3, 90-4.
Farideh, N., Rosfarizan, M., Javad, B., 2013. Antioxidant, Antiproliferative effects of Polyphenol rich Seaweed (Sargassummuticum). BioMed. Research. International. 604787, 9.
Liyan, S., Shengfang, R., Rongmin, Y., Chunyan, Y., Tingfei, L., Yu, Z., 2008. Purification, characterization and in vitro anti-tumor activity of proteins from arca subcrenata lischke. Marine. Drugs. 6, 418-30.
Harada, H., Noro, T., Kamei, Y., 1997. Selective antitumor activity in vitro from marine algae from Japan coast. Biol. Pharm. Bull. 20, 541-6.
Scudiero, D., 1988. Evaluation of a soluble tetrazolium/formazan assay for cell growth & drug sensitivity in culture using human and other cell lines. Cancer. Research. 48, 4827-4833.
Neal, W.R., George, H.R., Stephen, M.H., Andrew, L.G., 1991. An improved colorimetric assay for cell proliferation and viability utilizing the tetrazolium salt XTT. J. Immunol. Methods. 142, 257-265.
Ayesha., Hira., Sultana, V., Ara, J., Ehteshamul-Haque, S., 2010. In vitro cytotoxicity of seaweeds from Karachi coast on brine shrimp. Pak. J. Bot. 42, 3555-60.
Zandi, K., Ahmadzadeh, S.S., 2010. Anticancer activity of Sargassum oligocystum water extract against human cancer cell lines. Eur. Rev. Med. Pharmacol. Sci. 14, 669-673.
Sobhani., Alireza., et al., 2015. Antioxidant Effects of Brown Algae Sargassum on Sperm Parameters. Medicine. 94, 52-58.
Stella, M.J., Vinotha, P., Pradeep, M., 2012. Screening for In vitro Cytotoxic Activity of Seaweed, Sargassum sp. Against Hep-2 and MCF-7 Cancer Cell Lines. Asian. Pacific. J. Cancer. Prev. 13 (12), 6073-6076.
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