Development and study of wound healing potential of a polyherbal formulation

Abstract

Plants are more potent healers because they promote the repair mechanisms in the natural way. Wound healing potential of a polyherbal formulation consisting of Calophyllum inophyllum L, Hibiscus sabdariffa L and Quercus infectoria Olive in Excision, Incision and Dead space wounded rat models and also compared with reference ointment. Wistar strain of albino rats weighing 150 – 200g has been divided into 9 groups each comprising of 6 rats. Group – I, IV and VII served as wounded control, group – II, V and VIII are wounded and treated with PHO topically, group – III, VI and IX are wounded and treated with reference drug.  The biochemical and enzymatic parameters studied were hydroxyproline, hexosamine, ascorbic acid, superoxide dismutase, lipid peroxide and protein, DNA, RNA and WBC. These parameters were increased in the rats treated with polyherbal ointment. Lipid peroxide and WBC level was decreased when compared to control and standard group.  The results of the present study substantiate the traditional claims that the use of PHO prepared from plants possesses significant wound healing promoting activity and provides scientific evidence to the ethnomedicinal property of plants in the healing of wounds.