Antioxidant activity of some wild mushrooms from southern Western Ghats, India

Ragupathi, V; Stephen, A; Arivoli, D; Kumaresan, S

Authors

Ragupathi, V PG and Research Department of Botany, Ramakrishna Mission Vivekananda College (Autonomous), Mylapore, Chennai – 600004, India.
Stephen, A 1. PG and Research Department of Botany, Ramakrishna Mission Vivekananda College (Autonomous), Mylapore, Chennai – 600004, India. 2. Department of Pothu Maruthuvam,
Arivoli, D Department of Pothu Maruthuvam, Govt. Siddha Medical College & Hospital, Palayamkottai, Tirunelveli – 627002, India.
Kumaresan, S PG and Research Department of Botany, Ramakrishna Mission Vivekananda College (Autonomous), Mylapore, Chennai – 600004, India.

Keywords:

Antioxidant activity, macrofungi, Western Ghats, Gymnopilus junonius, Tricholoma equestre

Abstract

Objective: To investigate the antioxidant activities of crude methanol extracts of 10 southern Western Ghats wild mushrooms.

Methods: Crude methanol extracts from 10 mushrooms from southern Western Ghats, India were evaluated for their antioxidant activity by Nitric Oxide scavenging assay and DPPH scavenging assay.

Results: The methanol extract of Trametes versicolor recorded maximum percentage of NO activity of 94% followed by Tricholoma equestre 92% at the concentration 50 µg/ml. However, the extract of Gymnopilus junonius (80.33%) and Tricholoma equestre (79%) tested against DPPH stable radicals reveals that the radical scavenging activity of wild mushrooms possessed excellent antioxidant capacity which increased with the increasing concentration of the extract.

Conclusions: Gymnopilus junonius, Tricholoma equestre and Trametes versicolor have higher antioxidant activity showed both in Nitric Oxide scavenging assay and DPPH scavenging assay.

Downloads

Download data is not yet available.

References

Selim KA, El-Beih AA, AbdEl-Rahman TM, El-Diwany AI. Biology of Endophytic Fungi. Curr. Res. Environ. Appl. Mycol. 2012; 2(1):31-82.

Zhong JJ, Xiao JH. Secondary metabolites from higher fungi: Discovery, bioactivity and biopro-duction. Adv. Biochem. Eng. Biotechnol. 2009; 113:79-150.

Shen J, Xu X, Cheng F, Liu H, Luo X, Shen J. et al., Virtual screening on natural products for discovering active compounds and target information. Curr. Med. Chem., 2003; 10(21):2327-42.

Gaston KJ. 2000. Global patterns in biodiversity. Nature 2000; 405:220-227.

Hawksworth DL, Kalin-Arroyo MT. Magnitude and distribution of biodiversity. In: Heywood VH. (ed). Global Biodiversity Assessment. United Nations Environment Programme, p. 107-192. 1995.

O’Brien BL, Parrent JL, Jackson JA, Moncalvo JM, Vilgalys R. Fungal community analysis by large-scale sequencing of environmental samples. Appl. Environ. Microbiol. 2005; 71:5544-5550.

Taylor DL, Herriott IC, Stone KE, McFarland JW, Booth MG, Leigh MB. Structure and resilience of fungal communities in Alaskan boreal forest soils. Can. J. For. Res. 2010; 40:1288-1301.

Blackwell M. The Fungi: 1, 2, 3 … 5.1 million species? Am. J. Bot. 2011; 98(3):426-438.

Sarbhoy AK, Agarwal DK, Varshne JL. Fungi of India 1982-1992. CBS Publishers and distributors, New Delhi. 1996.

Kock JLF, Strauss T, Pohl CH, Smith DP, Botes PJ, Pretorius EE. et al., Bioprospecting for novel oxylipins in fungi: the presence of 3-hydroxy oxylipins in Pilobolus. Antonie van Leeuwenhoek. 2001; 80:93-99.

Bode HB, Bethe B, Höfs R, Zeek A. Big effects from small changes: possible ways to explore nature?s chemical diversity. ChemBioChem. 2002; 3:619-627.

Donadio S, Monicardini P, Alduina R, Mazzaa P, Chiocchini C, Cavaletti L. et al., Microbial technologies for the discovery of novel bioactive metabolites. J. Biotechnol. 2002; 99:187-198.

Chin YW, Balunas MJ, Chai HB, Kinghorn AD. Drug discovery from natural sources. AAPS J. 2006; 8:239-253.

Gunatilaka AAL. Natural products from plant-associated microorganisms: Distribution, structural diversity, bioactivity, and implications of their occurrence. J. Nat. Prod. 2006; 69:509-526.

Mitchell AM, Strobel GA, Hess WM, Vargas PN, Ezra D. Muscodor crispans, a novel endophyte from Anans ananassoides in the Bolivian Amazon. Fungal Divers. 2008; 31:37-43.

Stadler M, Keller NP. Paradigm shifts in fungal secondary metabolite research. Mycol. Res. 2008; 112:127-130.

Wasser SP, Weis LA. 1999. Medicinal properties of substances occurring in higher Basidiomycete mushroom: current perspective. Int. J. Med. Mushrooms. 1999; 1:31-62.

Lindequist U, Niedermeyer THJ, Jülich WD. The pharmacological potential of mushrooms. eCAM. 2005; 2:285-299.

Ajith TA, Janardhanan KK. Indian Medicinal Mushrooms as a Source of Antioxidant and Antitumor Agents. J. Clin. Biochem. Nutr. 2007; 40:157-162.

Thatoi H, Singdevsachan SK. Diversity, nutritional composition and medicinal potential of Indian mushrooms: A review. Afr. J. Biotechnol. 2014; 13(4):523-545.

Blois MS. Antioxidant determinations by the use of a stable free radical, Nature. 1958; 181:1199-1200.

Furchgott RF, Zawadzki JV. The obligatory role of endothelial cells in the relaxation of arterial smooth muscle by acetylcholine. Nature, 1980; 288(5789):373-376.

Snyder SH, Bredt DS. Biological roles of nitric oxide. Sci. Am. 1992; 266:68-77.

Moncada S, Higgs A. The L-arginine-nitric oxide pathway. N. Engl. J. Med. 1993; 329:2002-2012.

Nathan C, Xie Q. Nitric oxide synthases: roles, tolls, and controls. Cell. 1994; 78:915-918.

Kelm M, Dahmann R, Wink DA, Feelisch M. A new method for the simultaneous evaluation of rates of NO and O2- formation. Insights into the NO/O2- chemistry. J. Biol. Chem. 1997; 272:9922-9932.

Acharya K, Yonzone P, Rai M, Acharya R. Antioxidant and nitric oxide synthase activation properties of Ganoderma applanatum. Indian J. Exp. Biol. 2005; 43:926-929.

Boora F, Chirisa E, Mukanganyama S. Evaluation of Nitrite Radical Scavenging Properties of Selected Zimbabwean Plant Extracts and Their Phytoconstituents. J. Food Process. 2014; 2014(2014), Article ID 918018, 7 pages.

Amarowicz R, Pegg RB, Rahimi-Moghaddam P, Barl B, Weil JA. Free-radical scavenging capacity and antioxidant activity of selected plant species from the Canadian prairies. Food Chem. 2004; 84:551-562.

Ferreira ICFR, Baptista P, Vilas-Boas M, Barros L. Free-radical scavenging capacity and reducing power of wild edible mushrooms from northeast Portugal: Individual cap and stipe activity. Food Chem. 2007; 100:1511-1516.

Wang HX, Ooi VEC, Ng TB, Chiu KW, Chang ST. Hypotensive and vasorelaxing activities of a lectin from the edible mushroom Tricholoma mongolicum. Pharmacol. Toxicol. 1996; 79:318-323.

Acharya K, Samui K, Rai M, Dutta BB, Acharya R. Antioxidant and nitric oxide synthase activation properties of Auricularia auricula. Indian J. Exp. Biol. 2004; 42:538-540.

Guerra-Dore CMP, Azevedo TCG, de Souza MCR, Rego LA, de Dantas JCM, Silva FRF. et al., Antiinflam-matory, antioxidant and cytotoxic actions of ?-glucanrich extract from Geastrum saccatum mushroom. Int. Immunopharmacol., 2007; 7(Suppl 9): 1160-1169.

Barros L, Cruz T, Baptista P, Estevinho LM, Ferreira ICFR. Wild and commercial mushrooms as source of nutrients and nutraceuticals. Food Chem. Toxicol. 2008; 46(Suppl 8): 2742-2747.

Menaga D, Rajakumar S, Ayyasamy PM. Free radical scavenging activity of methanolic extract of Pleurotus florida mushroom. Int. J. Pharm. Pharm. Sci., 2013; 5(Suppl 4): 601-606.

Jayakumar T, Thomas PA, Sheu JR, Geraldine P. In-vitro and invivo antioxidant effects of the oyster mushroom Pleurotus ostreatus. Food Res. Int. 2011; 44:851-861.

Nitha B, De S, Adhikari SK, Devasagayam TP, Janardhanan KK. Evaluation of free radical scavenging activity of morel mushroom, Morchella esculenta mycelia: a potential source of therapeutically useful antioxidants. Pharm. Biol., 2010; 48(4):453-460.

Premkumari B, Shivashankar M. Study on invitro free radical scavenging activity of Hypsizygus ulmarius mushroom. J. Chem. Pharm. Res. 2014; 6(6):501-507.

Alispahi? A, Šap?anin A, Salihovi? M, Rami? E, Dedi? A, Pazalja M. Phenolic content and antioxidant activity of mushroom extracts from Bosnian market. Bull. Chem. Technol. Bosnia Herzegovina. 2015; 44:5-8.

Sumathy R, Ajesh TP, Kumuthakalavalli R. Dpph Free Radical Scavenging Activity and Total Phenolic Content of Three Species of Oyster Mushrooms. Biotechnol. 2013; 3(10):1-3.

Kele? A, Koca ?, Gençcelep H. Antioxidant Properties of Wild Edible Mushrooms. J. Food Process. Technol. 2011; 2:130.

Huang SJ, Huang LC, Chen CC, Mau JL. Antioxidant properties of Agaricus blazei. In: Broderick A, Nair T. (Eds.), Proceedings of the third international conference on mushroom biology and mushroom products (266- 274), Sydney, Australia. 1999.

Tsai SY, Tsai HL, Mau JL. Antioxidant properties of Agaricus blazei, Agrocybe cylindracea, and Boletus edulis. LWT-Food Sci. Technol. 2007; 40:1392-1402.

Tsai SY, Tsai HL, Mau JL. Antioxidant properties of Coprinus comatus. J. Food Biochem. 2009; 33(3):368-389.

Lee YL, Huang GW, Liang ZC, Mau JL. Antioxidant properties of three extracts from Pleurotus citrinopileatus. LWT-Food Sci. Technol. 2007a; 40:823-833.

Lee YL, Yen MT, Mau JL. Antioxidant properties of various extracts from Hypsizigus marmoreus. Food Chem. 2007b; 104:1-9.

Sharma SK, Gautam N. Chemical, Bioactive, and Antioxidant Potential of Twenty Wild Culinary Mushroom Species. Biomed Res. Int. 2015: Article ID 346508.

Antioxidant activity of some wild mushrooms from southern Western Ghats, India

Authors

Keywords:

Abstract

Downloads

References

Published

How to Cite

Issue

Section

Information