Analysis of Cytogenetic Alterations in Patients affected by Hepatocellular Carcinoma in Tamilnadu Population

Sasikala P; Vasanthakumar S; Venkatesh B; Padma M; Sukumar C; Balachandar V; Ganesan S

Authors

Sasikala P PG & Research Department of Zoology and Biotechnology, A.V.V.M. Sri Pushpam College (Autonomous), Poondi, Thanjavur-613503, Tamil Nadu, India
Vasanthakumar S PG & Research Department of Zoology and Biotechnology, A.V.V.M. Sri Pushpam College (Autonomous), Poondi, Thanjavur-613503, Tamil Nadu, India
Venkatesh B Human Molecular Cytogenetics and Stem Cell Laboratory, Department of Human Genetics and Molecular Biology, Bharathiar University, Coimbatore – 641046, Tamil Nadu, India
Padma M PG & Research Department of Zoology and Biotechnology, A.V.V.M. Sri Pushpam College (Autonomous), Poondi, Thanjavur-613503, Tamil Nadu, India
Sukumar C Human Molecular Cytogenetics and Stem Cell Laboratory, Department of Human Genetics and Molecular Biology, Bharathiar University, Coimbatore – 641046, Tamil Nadu, India
Balachandar V Human Molecular Cytogenetics and Stem Cell Laboratory, Department of Human Genetics and Molecular Biology, Bharathiar University, Coimbatore – 641046, Tamil Nadu, India
Ganesan S PG & Research Department of Zoology and Biotechnology, A.V.V.M. Sri Pushpam College (Autonomous), Poondi, Thanjavur-613503, Tamil Nadu, India

Keywords:

Hepatocellular Carcinoma (HCC), Chromosomal aberrations (CAs), deletion, Tamil Nadu

Abstract

The Hepatocellular carcinoma is one of the most lethal cancers from all of the gastrointestinal tract cancer. In HCC the involvement of chromosome 1 and 3 mainly in the region of 1p36.11 particularly in the short arm have a predominant alteration.
The objective of this study is to find the region of the altered chromosome and to find the novel biomarker for cytogenetic analysis of HCC cancer in and around the Tamilnadu state. In the present study, totally 10 Peripheral blood samples from
HCC cancer patients were obtained. The chromosomal banding is performed by the Trypsin- Giemsa banding technique and the microscope slides were evaluated under the microscope by 100x magnification and statistical data is processed by ANOVA. Further in future we can go for the FISH technique, by specifically we can use the particular probes for the identification of the altered region in chromosome of HCC patients.