DEVELOPMENT AND VALIDATION OF ANALYTICAL METHOD FOR SIMULTANEOUS ESTIMATION OF TENO-FOVIR AND EMTRICITABINE IN PHARMACEUTICAL DOSAGE FORMS BY HPLC

Authors

  • Z. M. Sayyed Department of Quality Assurance, Anuradha College of Pharmacy, Chikhli, Dist.-Buldana, Pin - 443201, Maharashtra (India).
  • Aijaz A. Sheikh Department of Quality Assurance, Anuradha College of Pharmacy, Chikhli, Dist.-Buldana, Pin - 443201, Maharashtra (India).
  • Zakirhussain A. Shaikh B.Sci. (PCM), Siddharth Sci. & Jr. College, Jafrabad, Dist.- Jalna, Pin- 431206, Maharashtra (India).
  • S. A. Shinde Department of Quality Assurance, Anuradha College of Pharmacy, Chikhli, Dist.-Buldana, Pin - 443201, Maharashtra (India).
  • . J. Chaware5 Department of Quality Assurance, Anuradha College of Pharmacy, Chikhli, Dist.-Buldana, Pin - 443201, Maharashtra (India).
  • K. R. Biyani Department of Quality Assurance, Anuradha College of Pharmacy, Chikhli, Dist.-Buldana, Pin - 443201, Maharashtra (India).

Keywords:

Tenofovir, Emtricitabine, Simultaneous Estimation, Validation, HPLC

Abstract

A simple, accurate, rapid and precise High performance Liquid Chromatography (HPLC) method was developed and validated for the simultaneous estimation of  Tenofovir (TFR) and Emtricitabine  (ECB) in combined tablet dosage form.  Chromatogram was run through column of Hi Q C18 W (150 mm: 4.6 mm, 5 µ). Mobile phase containing Buffer, Methanol and Acetonitrile (40: 50: 10) was pumped through column at a flow rate of 1.3 ml/ min. 0.01 N KH2PO4  was used as Buffer. UV detection was carried out at 265 nm and 278 nm for Tenofovir and Emtricitabine respectively. Retention times of Tenofovir and Emtricitabine were found to be  4.38 min and 3.22 min respectively. % RSD of the Tenofovir and Emtricitabine was found to be less than 2 %. The Calibration curves were plotted in the concentration range of 6-36 µg/ ml for Tenofovir and 5-30 µg/ml for Emtricitabine with R2= 0.99. % Recovery was found between 97-100 %  for both the drugs.

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References

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Published

2016-01-15
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How to Cite

Z. M. Sayyed, Aijaz A. Sheikh, Zakirhussain A. Shaikh, S. A. Shinde, . J. Chaware5, and K. R. Biyani. “DEVELOPMENT AND VALIDATION OF ANALYTICAL METHOD FOR SIMULTANEOUS ESTIMATION OF TENO-FOVIR AND EMTRICITABINE IN PHARMACEUTICAL DOSAGE FORMS BY HPLC”. International Journal of Pharmaceutics and Drug Analysis, vol. 4, no. 1, Jan. 2016, pp. 24-29, https://ijpda.org/index.php/journal/article/view/191.

Issue

Volume-4, Issue-1, January-2016

Section

Research Articles
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