A CONCISE SYSTEMATIC REVIEW ON LIQUID CHROMATOGRAPHY/ MASS SPECTROMETRY WITH SPECIAL EMPHASIS ON ITS APPLICATIONS

Abstract

Mass spectrometry (MS) is a wide-ranging analytical tool, which involves the production and subsequent separation and identification of charged species. MS provides valuable information of molecular mass, molecular structural information and
quantitative data all at high sensitivity. However, it is best separation techniques applied to complex mixtures before mass spectrometry is undertaken. Liquid chromatography (LC) is excellent for separating mixtures but generally poor at identification of compounds. It is an arrangement of two or more mass spectrometers tandem-in-space and time placed one behind the other.
Literature reveals many analytical approaches with detection systems, automation tools for an effective separation, enhanced selectivity and sensitivity for quantitation of many analytes. The bioanalytical method (s) development plays an important role during the process of drug discovery and development culminating in a marketing approval. The intention of this review is to cover various key areas where LC/MS/MS shows its applicability in bioanalytical areas.