INHIBITION OF BACTERIAL PATHOGENS OF TROPICAL TASAR SILKWORM (ANTHEREAE MYLITTA D.) BY USING FOLIOSE LICHENS ISOLATED FROM SAL PLANT (SHOREA ROBUSTA).

Madhusudhan, K.N; Nisha-Kachhap; A.K. Sinha Singh; G.P., Gupta; V.P., Naqvi A.H; Alok Sahay

Authors

Madhusudhan, K.N Department of Microbiology, Central Tasar Research and Training Institute, Piska Nagri, Ranchi-835303, Jharkhand, INDIA
Nisha-Kachhap Department of Microbiology, Central Tasar Research and Training Institute, Piska Nagri, Ranchi-835303, Jharkhand, INDIA
A.K. Sinha Singh Department of Microbiology, Central Tasar Research and Training Institute, Piska Nagri, Ranchi-835303, Jharkhand, INDIA
G.P., Gupta Department of Microbiology, Central Tasar Research and Training Institute, Piska Nagri, Ranchi-835303, Jharkhand, INDIA
V.P., Naqvi A.H Department of Microbiology, Central Tasar Research and Training Institute, Piska Nagri, Ranchi-835303, Jharkhand, INDIA
Alok Sahay Department of Microbiology, Central Tasar Research and Training Institute, Piska Nagri, Ranchi-835303, Jharkhand, INDIA

Keywords:

Tasar silkworm, Bacterial diseases, Lichens, Antibacterial activity, TLC, FTIR

Abstract

Bacterial diseases are causing considerable yield loss to tropical tasar silkworm (A. mylitta D.) during rearing. The presently common chemical disinfectants are being used for the control of bacterial pathogens. Lichens are potent antimicrobial compounds which are being exploited in different fields. Since, abundant presence of lichens was noticed in the tasar rearing plots, the present study was aimed to exploit the locally available lichens as antibacterial agents. The foliose lichen was collected from S. robusta plant and used in the present study. The lichens showed very promising results by inhibition pathogenic bacteria species infecting tropical tasar silkworm. The in vitro inhibition results revealed that, methanol and ethanol extract of lichens showed more zone of inhibition in comparison with other treatments and control. The separation of compounds by using TLC revealed the presence of different compounds at different RF value in both methanol and ethanol extract. The FTIR analysis of methanol and ethanol extract showed the presence of different functional groups in the extracts. Amino and alkene groups
are responsible for the antimicrobial activity. Based on the results of the present study, the lichens can be used as potent biocontrol agent against bacterial pathogens infecting tropical tasar silkworm.