DEVELOPMENT AND VALIDATION OF REVERSE PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY METHOD FOR ESTIMATION OF BLONANSERIN IN SYNTHETIC MIXTURE

Abstract

This research manuscript describes simple, sensitive, accurate, precise and repeatable reverse phase high
performance liquid chromatography method for the determination of Blonanserin in synthetic mixture. The sample was analyzed by reverse phase C18 column (Phenomenex C18, 250 mm × 4.6 mm, 5?) as stationary phase; acetonitrile : methanol : phosphate buffer, pH 3.0 (10 : 70 : 20 , v/v/v) as a mobile phase at a flow rate of 1.0 ml/min. Quantification was achieved with Photo Diode Array detector at 238 nm. The retention time for Blonanserin was found to be 5.11 min. The linearity for the drug was obtained in the concentration range of 1-8 ?g/ml with mean accuracies 99.89 ± 0.79. The method was successfully applied to synthetic mixture because no chromatographic interferences from formulation excipients were found. The method retained its accuracy and precision when the standard addition technique was applied.