IN VITRO ANTIOXIDANT ACTIVITY OF VARIOUS EXTRACTS OF WHOLE PLANT OF PERISTROPHE PANICULATA FORSSK

Israel raja johnley; Prabal joshi

Authors

Israel raja johnley Department of pharmacology, Mahatma Gandhi Medical college and Research Institute, Pondicherry, India
Prabal joshi Department of Physiology,SGT Medical college , Gurgaon, Haryana, India

Keywords:

Whole plant of Peristrophe paniculata, In vitro antioxidant, DPPH assay, Superoxide anion,flavonoids

Abstract

The objective of this investigation was to evaluate the in vitro antioxidant activities of various extracts of whole plant of Peristrophe paniculata . All the three extracts were examined for DPPH (-diphenyl-?-picrylhydrazyl) radical scavenging activity, Superoxide Anion Scavenging Activity with reference standard Rutin and Quercetin respectively through in vitro
models and estimate the total flavonoids. The methanolic extract of Peristrophe paniculata was found to most active than that of other two extracts of Peristrophe paniculata scavenging DPPH radicals with Rutin was used as reference standard and an IC50 value was found to be 612?g/ml and 480?g/ml respectively. Both the method significantly showed the
methanolic extract of Peristrophe paniculata was found to most effective than that of petroleum ether & ethyl acetate extract. The methanolic extract of Peristrophe paniculata was found higher content of flavonoids than that of petroleum ether and ethyl acetate. It is concluded that the methanolic extract of Peristrophe paniculata showed strong scavenging
activity against free radical compared to all other extracts.