DEVELOPMENT AND VALIDATION OF RP-HPLC METHOD FOR SIMULTANEOUS ESTIMATION OF RISPERIDONE AND HALOPERIDOL IN TABLET DOSAGE FORMS

Abstract

A new, simple and sensitive reverse phase high performance liquid chromatographic (RP-HPLC) method has been
developed for the separation and quantification of Risperidone(RIS) and haloperidol (HPD) in tablet dosage form. The
determination was carried out using XBridge C18 [4.6 x 150 mm] column as a stationary phase and mobile phase comprised
of Methanol: triethyl amine Buffer (60::40) and the pH of triethylamine adjusted to pH2.5 using orthophosphoric acid. The
flow rate was maintained at 1.0 ml/min and the eluent was monitored at 260nm.The retention time of RIS and HPD were
1.82 min and 4.42min respectively. The method was validated in terms of linearity, precision, accuracy, specificity and
robustness. The method was linear and for precision studies; RSD for RIS AND HPD were 0.02 and 0.04 respectively. The
percentage recoveries for both drugs from their tablets were 100.80 and 99.76 % respectively.