RP-HPLC APPROACH FOR CONCURRENT QUANTIFICATION OF TRIFLURIDINE AND TIPIRACIL PHARMACEUTICAL AND IN BULK FORMS: DEVELOPMENT AND VALIDATION

Authors

  • B.Mallikarjuna Department of Chemistry, Government College (A), Adikavi Nannaya University, Rajahmundry, E.G Dt., A.P, India.

DOI:

https://doi.org/10.47957/ijpda.v12i3.601

Keywords:

Symmetry C18, tipiracil and trifluridine, RP-HPLC

Abstract

The proposed RP-HPLC approach effectively quantifies tipiracil and trifluridine in pharmaceutical dosage forms, offering high sensitivity, linearity, and repeatability. Using a phosphate based buffer pH 4.6 and a carrier liquid of methanol with phosphate buffer solution (70:30 v/v), the method employs a Symmetry C18 column (4.6 x 150mm, 5?m, XTerra) with UV absorbance measurement at 273 nm ,the flow capacity of 1.0 ml/min and the  run time was carried for 7mins. The retention duration of Tipracil is 2.003min and 5.067min for Trifluridine. The linearity range is 25-125 ?g/ml for both drugs while the regression coefficient lies at  ? 0.999. The precision of developed method  is confirmed by %RSD values under 2%, and recovery rates of 97-102% demonstrate accuracy and minimal excipient interference, making it suitable for routine quality control.

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References

REFERENCES

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Published

2024-10-09
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How to Cite

B, M. . “RP-HPLC APPROACH FOR CONCURRENT QUANTIFICATION OF TRIFLURIDINE AND TIPIRACIL PHARMACEUTICAL AND IN BULK FORMS: DEVELOPMENT AND VALIDATION”. International Journal of Pharmaceutics and Drug Analysis, vol. 12, no. 3, Oct. 2024, pp. 34-41, doi:10.47957/ijpda.v12i3.601.

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