Validated Spectrophotometric Method for the Determination of Paracetamol and Tamadol Hydrochloride in Tablet dosage form

Abstract

A simple, precise and accurate method was developed for the estimation of Paracetamol (PCM) and Tramadol
Hydrochloride (TRM) in tablet dosage form using second order derivative spectrophotometry. Wavelengths selected for
quantitation were 264 nm for Paracetamol (zero crossing point of TRM) and 224.06 nm for Tramadol Hydrochloride (zero
crossing point of PCM). The method was validated with respect to linearity, accuracy, precision, limit of detection and limit
of quantitation in accordance with the international conference on harmonisation (ICH) guidelines. Linearity was observed
in concentration range of 2-14 ?g/ml for each Paracetamol and Tramadol Hydrochloride. The limit of detection and limit of
quantitation were found to be 0.396 ?g/ml and 1.2 ?g/ml for Paracetamol while 0.429 ?g/ml and 1.3 ?g/ml for Tramadol
Hydrochloride, respectively. The percentage recovery of Paracetamol and Tramadol Hydrochloride was found to be 99.29 ±
0.57 and 99.61 ± 0.29, respectively. The % R.S.D. values for intra-day and inter-day precision study were <2.0%,
confirming that the method was precise. The method can be successfully employed for the simultaneous estimation of
Paracetamol and Tramadol Hydrochloride in tablet dosage form.