Determination of phenelzine in human plasma sample using SPE- UPLC–MS/MS assay
Keywords:
Phenelzine, human plasma, UPLC, LC-MS/MS, solid Phase extraction, validationAbstract
In this paper a fast and highly sensitive ultra-high performance liquid chromatography (UPLC) method for the determination of phenelzine in human plasma have been developed using tandem mass spectrometry (MS/MS) detection. Hydroxyzine was used as an internal standard (IS). The extraction of the phenelzine from human plasma was performed using solid phase extraction. ACE-C18 (5µm, 100 x 4.6mm) reverse phase column was employed for chromatographic separation of analyte and internal standard for MS/MS detection at 0.9 ml/min flow. Detection was performed at transitions of m/z 137.258? 106.906 for phenelzine and m/z 376.022? 202.006 for hydroxyzine by positive electro-spray ionization (ESI+) in multiple reaction monitoring (MRM) mode using tandem mass spectrometry. The developed method was compared in the terms of validation parameters including linearity, sensitivity, precision and accuracy. The analysis was carried out in 3.0 min and the matrix matched calibration curves in the range of 0.508 ng/mL to 25.144 ng/mL were used for quantification with the correlation coefficients demonstrating good linearity (0.994-0.999). The mean extraction recoveries for phenelzine and IS from plasma were 96.5 % and 95.3% respectively. Matrix based samples were stable at room temperature for 12 hrs, processed samples were stable at least for 28 hrs and also stable at six freeze-thaw cycles. This method was successfully applied for determination of phenelzine in human plasma for pharmacokinetic study.
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