VALIDATED RP – HPLC METHOD FOR SIMULTANEOUS ESTIMATION OF TELMISARTAN AND AMLODIPINE

Abstract

A simple, sensitive and rapid reverse phase HPLC method was developed for the simultaneous estimation of Telmisartan
and Amlodipine. AnHypersil BDS 100mm x 4.6 mm, 5? column was used. The mobile phase consisting of Phosphate
buffer (pH 3.5): Acetonitrile taken in the ratio 57: 43 that was set at a flow rate of 1.0 mL/min, the column temperature was
maintained at 30ºC and the measurements were made at 237 nm. The retention times of Telmisartan and Amlodipine were
found to be 2.560 and 3.148 min, respectively.The validation of the proposed method was carried out for its specificity,
accuracy, precision, linearity, limit of detection and limit of quantification for both Amlodipine and Telmisartan.